Ultrasound-Assisted Extraction of Protein from Pumpkin Seed Press Cake: Impact on Protein Yield and Techno-Functionality

Research output: Contribution to journalResearch articleInvitedpeer-review

Contributors

Abstract

Conventional solvent-based methods widely used for isolating plant proteins may deliver an unsatisfactory protein yield and/or result in protein degradation. The present study started with the optimization of pumpkin seed protein from press cake by alkaline extraction and subsequent isoelectric precipitation. Subsequently, extraction was supported by ultrasound under three conditions: ultrasonic treatment followed by alkaline extraction (US+AE), concomitant ultrasonic treatment and alkaline extraction (UAE), and alkaline extraction followed by ultrasonic treatment (AE+US). Compared to the control group, an increase in protein yield was achieved after ultrasonic treatment, while the highest protein yield was obtained with AE+US (57.8 ± 2.0%). Isolates with a protein content of 94.04 ± 0.77 g/100 g and a yield of 43.6 ± 0.97% were obtained under optimized conditions. Following ultrasonic treatment applied during extraction, solubility, foaming capacity, foam stability, and denaturation enthalpy of the isolated protein increased, and water binding capacity decreased as compared to non-sonicated samples. The d90 particle size percentile of the extracted suspensions was 376.68 ± 38.32 µm for the control experiments, and particle size was significantly reduced in ultrasound-assisted treatments down to d90 = 179.93 ± 13.24 µm for the AE+US treatment). Generally, ultrasonication resulted in a significant increase in protein yield and improved techno-functional properties of the isolates.

Details

Original languageEnglish
Article number4029
JournalFoods
Volume11
Issue number24
Publication statusPublished - 13 Dec 2022
Peer-reviewedYes

External IDs

PubMed 36553771
Scopus 85144734220
WOS 000902710700001
ORCID /0000-0002-1281-5966/work/142248837

Keywords

Keywords

  • alkaline extraction, foaming capacity, foaming stability, solubility, ultrasonic treatment, Alkaline extraction, Foaming stability, Foaming capacity, Solubility, Ultrasonic treatment

Library keywords