Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
In contrast to CRISPR/Cas9 nucleases, CRISPR base editors (BE) and prime editors (PE) enable predefined nucleotide exchanges in genomic sequences without generating DNA double strand breaks. Here, we employed BE and PE mRNAs in conjunction with chemically synthesized sgRNAs and pegRNAs for efficient editing of human induced pluripotent stem cells (iPSC). Whereas we were unable to correct a disease-causing mutation in patient derived iPSCs using a CRISPR/Cas9 nuclease approach, we corrected the mutation back to wild type with high efficiency utilizing an adenine BE. We also used adenine and cytosine BEs to introduce nine different cancer associated TP53 mutations into human iPSCs with up to 90% efficiency, generating a panel of cell lines to investigate the biology of these mutations in an isogenic background. Finally, we pioneered the use of prime editing in human iPSCs, opening this important cell type for the precise modification of nucleotides not addressable by BEs and to multiple nucleotide exchanges. These approaches eliminate the necessity of deriving disease specific iPSCs from human donors and allows the comparison of different disease-causing mutations in isogenic genetic backgrounds.
Details
Original language | English |
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Article number | 511 |
Journal | Genes |
Volume | 11 |
Issue number | 5 |
Publication status | Published - 6 May 2020 |
Peer-reviewed | Yes |
External IDs
PubMedCentral | PMC7288465 |
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ORCID | /0000-0001-9335-9749/work/116692782 |
Scopus | 85084579488 |
Keywords
Sustainable Development Goals
Keywords
- Adenine/chemistry, Aminohydrolases, Autoimmune Diseases of the Nervous System/genetics, Base Sequence, CRISPR-Associated Protein 9, CRISPR-Cas Systems, Cell Line, Cellular Reprogramming Techniques, Cytosine/chemistry, Embryoid Bodies, Gene Editing/methods, Genes, p53, HEK293 Cells, Humans, Imidazoles/pharmacology, Induced Pluripotent Stem Cells/metabolism, Mutagenesis, Mutation, Nervous System Malformations/genetics, Piperazines/pharmacology, RNA, Guide/genetics, RNA, Messenger/genetics, SAM Domain and HD Domain-Containing Protein 1/genetics, Transfection