The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

RNA interference (RNAi) screens have been shown to be valuable to study embryonic stem cell (ESC) self-renewal and they have been successfully applied to identify coding as well as noncoding genes required for maintaining pluripotency. Here, we used an RNAi library targeting >640 long noncoding RNAs (lncRNA) to probe for their role in early cell differentiation. Utilizing a Sox1-GFP ESC reporter cell line, we identified the lncRNA lncR492 as lineage-specific inhibitor of neuroectodermal differentiation. Molecular characterization showed that lncR492 interacts with the mRNA binding protein HuR and facilitates its inhibitory function by activation of Wnt signaling. Thus, lncRNAs modulate the fate decision of pluripotent stem cells.

Details

Original languageEnglish
Article numbere0191682
JournalPloS one
Volume13
Issue number1
Publication statusPublished - 24 Jan 2018
Peer-reviewedYes

External IDs

PubMedCentral PMC5783419
Scopus 85041117695

Keywords

Keywords

  • Animals, Cell Differentiation, Embryonic Stem Cells/cytology, Mice, Neurons/cytology, RNA Interference, RNA, Long Noncoding/genetics, Reverse Transcriptase Polymerase Chain Reaction