Microbiome Characterization after Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
A demonstrator plant of a recently patented process for improved sludge degradation has been implemented on a municipal scale. In a 1500 m3 sewage sludge digester, an intermediary stage with aerobic sewage sludge reactivation was implemented. This oxic activation increased the biogas yield by up to 55% with a 25% reduction of the remaining fermentation residue volume. Furthermore, this process allowed an NH4-N removal of over 90%. Additionally, 16S rRNA gene amplicon high-throughput sequencing of the reactivated digestate showed a reduced number of methane-forming archaea compared to the main digester. Multiple ammonium-oxidizing bacteria were detected. This includes multiple genera belonging to the family Chitinophagaceae (the highest values reached 18.8% of the DNA sequences) as well as a small amount of the genus Candidatus nitrosoglobus (<0.3%). In summary, the process described here provides an economically viable method to eliminate nitrogen from sewage sludge while achieving higher biogas yields and fewer potential pathogens in the residuals.
Details
| Original language | English |
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| Article number | 471 |
| Journal | Fermentation |
| Volume | 9 |
| Issue number | 5 |
| Publication status | Published - May 2023 |
| Peer-reviewed | Yes |
External IDs
| ORCID | /0009-0006-1452-8801/work/151437264 |
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Keywords
ASJC Scopus subject areas
Keywords
- 16S rRNA sequencing, aerobic sludge activation, anaerobic digestion, anaerobic microbiomes, water treatment