Microbiome Characterization after Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Pascal Otto - , Chair of Waste Management and Circular Economy (Author)
  • Mozhdeh Alipoursarbani - , Brandenburg University of Technology Cottbus-Senftenberg (Author)
  • Daniel Torrent - , University of Valencia (Author)
  • Adriel Latorre-Pérez - , University of Valencia (Author)
  • Thomas Paust - , PRO-Entec East GmbH Bio Engineering (Author)
  • Alfred Albert - , PRO-Entec East GmbH Bio Engineering (Author)
  • Christian Abendroth - , Brandenburg University of Technology Cottbus-Senftenberg (Last author)

Abstract

A demonstrator plant of a recently patented process for improved sludge degradation has been implemented on a municipal scale. In a 1500 m3 sewage sludge digester, an intermediary stage with aerobic sewage sludge reactivation was implemented. This oxic activation increased the biogas yield by up to 55% with a 25% reduction of the remaining fermentation residue volume. Furthermore, this process allowed an NH4-N removal of over 90%. Additionally, 16S rRNA gene amplicon high-throughput sequencing of the reactivated digestate showed a reduced number of methane-forming archaea compared to the main digester. Multiple ammonium-oxidizing bacteria were detected. This includes multiple genera belonging to the family Chitinophagaceae (the highest values reached 18.8% of the DNA sequences) as well as a small amount of the genus Candidatus nitrosoglobus (<0.3%). In summary, the process described here provides an economically viable method to eliminate nitrogen from sewage sludge while achieving higher biogas yields and fewer potential pathogens in the residuals.

Details

Original languageEnglish
Article number471
JournalFermentation
Volume9
Issue number5
Publication statusPublished - May 2023
Peer-reviewedYes

External IDs

ORCID /0009-0006-1452-8801/work/151437264

Keywords

Keywords

  • 16S rRNA sequencing, aerobic sludge activation, anaerobic digestion, anaerobic microbiomes, water treatment