Imaging of nanoparticle-labeled stem cells using magnetomotive optical coherence tomography, laser speckle reflectometry, and light microscopy

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

Cell transplantation and stem cell therapy are promising approaches for regenerative medicine and are of interest to researchers and clinicians worldwide. However, currently, no imaging technique that allows three-dimensional in vivo inspection of therapeutically administered cells in host tissues is available. Therefore, we investigate magnetomotive optical coherence tomography (MM-OCT) of cells labeled with magnetic particles as a potential noninvasive cell tracking method. We develop magnetomotive imaging of mesenchymal stem cells for future cell therapy monitoring. Cells were labeled with fluorescent iron oxide nanoparticles, embedded in tissue-mimicking agar scaffolds, and imaged using a microscope setup with an integrated MM-OCT probe. Magnetic particle-induced motion in response to a pulsed magnetic field of 0.2 T was successfully detected by OCT speckle variance analysis, and cross-sectional and volumetric OCT scans with highlighted labeled cells were obtained. In parallel, fluorescence microscopy and laser speckle reflectometry were applied as two-dimensional reference modalities to image particle distribution and magnetically induced motion inside the sample, respectively. All three optical imaging modalities were in good agreement with each other. Thus, magnetomotive imaging using iron oxide nanoparticles as cellular contrast agents is a potential technique for enhanced visualization of selected cells in OCT.

Details

Original languageEnglish
Article number036018
JournalJournal of Biomedical Optics
Volume20
Issue number3
Publication statusPublished - 1 Mar 2015
Peer-reviewedYes

External IDs

Scopus 84926204131
Bibtex WOS:000352272300027
researchoutputwizard legacy.publication#65844
researchoutputwizard legacy.publication#65976
PubMed 25822955
researchoutputwizard legacy.publication#67049
ORCID /0000-0003-0554-2178/work/142249784
ORCID /0000-0002-0926-6556/work/142250468
ORCID /0000-0003-2292-5533/work/142256543
ORCID /0000-0001-9467-7677/work/161888168

Keywords

Keywords

  • cell imaging, laser speckle, magnetic nanoparticles, optical coherence tomography, regenerative medicine, stem cells