Foil-based DNA melting curve analysis platform for low-cost point-of-care molecular diagnostics

Research output: Contribution to book/Conference proceedings/Anthology/Report › Conference contribution › Contributed › peer-review

Contributors

  • A. Ohlander - , Fraunhofer Research Institution for Microsystems and Solid State Technologies (EMFT) (Author)
  • S. Bauer - , Fraunhofer Research Institution for Microsystems and Solid State Technologies (EMFT) (Author)
  • H. Ramachandraiah - , KTH Royal Institute of Technology (Author)
  • A. Russom - , KTH Royal Institute of Technology (Author)
  • K. Bock - , Chair of Electronic Packaging Technology, Fraunhofer Research Institution for Microsystems and Solid State Technologies (EMFT), Technical University of Berlin (Author)

Abstract

We report on genotyping of single nucleotide polymorphisms (SNP) by melting curve analysis (MCA) on DNA microarrays in a plastic microfluidic system with integrated heaters using lamination foils. Thin-film micro-heaters were processed on polyethylene napthalate (PEN) foil in only one metallization step. DNA microarrays were prepared directly on the PEN surface without the need of surface pre-treatment via UV-mediated immobilization of the spotted DNA microarrays. MCA in microfluidic channels is demonstrated at a ramping rate of 60°C/min, enabling ultra-rapid (50 seconds) SNP analysis.

Details

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society (CBMS)
Pages1770-1772
Number of pages3
Volume3
ISBN (print)9781632666246
Publication statusPublished - 2013
Peer-reviewedYes

Publication series

Series 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)

Conference

Title17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Duration27 - 31 October 2013
CityFreiburg
CountryGermany

External IDs

ORCID /0000-0002-0757-3325/work/139064938

Keywords

ASJC Scopus subject areas

Keywords

  • Lab-on-chip, Micro-heater, Microarray, Single nucleotide polymorphism