Foil-based DNA melting curve analysis platform for low-cost point-of-care molecular diagnostics
Publikation: Beitrag in Buch/Konferenzbericht/Sammelband/Gutachten › Beitrag in Konferenzband › Beigetragen › Begutachtung
Beitragende
Abstract
We report on genotyping of single nucleotide polymorphisms (SNP) by melting curve analysis (MCA) on DNA microarrays in a plastic microfluidic system with integrated heaters using lamination foils. Thin-film micro-heaters were processed on polyethylene napthalate (PEN) foil in only one metallization step. DNA microarrays were prepared directly on the PEN surface without the need of surface pre-treatment via UV-mediated immobilization of the spotted DNA microarrays. MCA in microfluidic channels is demonstrated at a ramping rate of 60°C/min, enabling ultra-rapid (50 seconds) SNP analysis.
Details
Originalsprache | Englisch |
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Titel | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
Herausgeber (Verlag) | Chemical and Biological Microsystems Society (CBMS) |
Seiten | 1770-1772 |
Seitenumfang | 3 |
Band | 3 |
ISBN (Print) | 9781632666246 |
Publikationsstatus | Veröffentlicht - 2013 |
Peer-Review-Status | Ja |
Publikationsreihe
Reihe | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013) |
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Konferenz
Titel | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 |
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Dauer | 27 - 31 Oktober 2013 |
Stadt | Freiburg |
Land | Deutschland |
Externe IDs
ORCID | /0000-0002-0757-3325/work/139064938 |
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Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- Lab-on-chip, Micro-heater, Microarray, Single nucleotide polymorphism