Space constraints govern fate of hematopoietic stem and progenitor cells invitro
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Deciphering exogenous cues that determine stem cell fate decisions is a persisting challenge of cell biology and bioengineering. In an effort to unravel the role of spatial constraints in the cell-instructive characteristics of bone marrow microenvironments, murine hematopoietic stem and progenitor cells (HSPC) were exposed to fibronectin-coated microcavities invitro. Microcavity sizes were chosen to allow for the inclusion of either individual or multiple cells. Repopulation experiments using lethally irradiated mice showed that the maintenance of functional HSPC in culture critically depends on cavity dimensions. Short-term repopulating hematopoietic stem cells (ST-HSC) were found to be best supported within single-cell sized compartments while long-term repopulating HSC (LT-HSC) were maintained within both cavity sizes. In sum, the reported data reveal spatial restriction to be a simple but powerful means for directing HSPC fate exvivo.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 709-715 |
Seitenumfang | 7 |
Fachzeitschrift | Biomaterials |
Jahrgang | 53 |
Publikationsstatus | Veröffentlicht - 1 Juni 2015 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 25890766 |
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ORCID | /0000-0003-0189-3448/work/161890457 |
Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- ECM, Invivo test, Microstructure, Stem cell