Space constraints govern fate of hematopoietic stem and progenitor cells invitro
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Deciphering exogenous cues that determine stem cell fate decisions is a persisting challenge of cell biology and bioengineering. In an effort to unravel the role of spatial constraints in the cell-instructive characteristics of bone marrow microenvironments, murine hematopoietic stem and progenitor cells (HSPC) were exposed to fibronectin-coated microcavities invitro. Microcavity sizes were chosen to allow for the inclusion of either individual or multiple cells. Repopulation experiments using lethally irradiated mice showed that the maintenance of functional HSPC in culture critically depends on cavity dimensions. Short-term repopulating hematopoietic stem cells (ST-HSC) were found to be best supported within single-cell sized compartments while long-term repopulating HSC (LT-HSC) were maintained within both cavity sizes. In sum, the reported data reveal spatial restriction to be a simple but powerful means for directing HSPC fate exvivo.
Details
Original language | English |
---|---|
Pages (from-to) | 709-715 |
Number of pages | 7 |
Journal | Biomaterials |
Volume | 53 |
Publication status | Published - 1 Jun 2015 |
Peer-reviewed | Yes |
External IDs
PubMed | 25890766 |
---|---|
ORCID | /0000-0003-0189-3448/work/161890457 |
Keywords
ASJC Scopus subject areas
Keywords
- ECM, Invivo test, Microstructure, Stem cell