Metabolic Transit of Dietary Methylglyoxal
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Methylglyoxal (MGO) is responsible for the pronounced antibacterial activity of manuka honey, in which it may reach concentrations up to 800 mg/kg. As MGO formed in vivo is discussed to play a role in diabetic complications, the metabolic transit of dietary MGO was studied within a 3 day dietary recall with four healthy volunteers. Determination of MGO in 24 h urine was performed with GC-MS after derivatization with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine, and D-lactate was quantified enzymatically. Following a diet virtually free from MGO and other glycation compounds, a defined amount of MGO (500 mu mol in manuka honey) was administered in the morning of day 2. Renal excretion was between 0.1 and 0.4 mu mol/day for MGO and between 50 and 220 mu mol/day for D-lactate. No influence on excretion of both compounds was observed following administration of MGO. To investigate the stability of MGO under physiological conditions, a simulated in vitro gastrointestinal digestion was performed with MGO-containing honey. After 8 h of in vitro digestion, only 5-20% of the initial methylglyoxal was recovered. This indicates that dietary MGO is rapidly degraded during the digestion process in the intestine and, therefore, exerts no influence on the MGO level in vivo.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 10253-10260 |
Seitenumfang | 8 |
Fachzeitschrift | Journal of Agricultural and Food Chemistry |
Jahrgang | 61 |
Ausgabenummer | 43 |
Publikationsstatus | Veröffentlicht - 30 Okt. 2013 |
Peer-Review-Status | Ja |
Externe IDs
Scopus | 84887120701 |
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WOS | 000326487700010 |
ORCID | /0000-0001-8528-6893/work/142256508 |
Schlagworte
Ziele für nachhaltige Entwicklung
Schlagwörter
- methylglyoxal, D-lactate, urinary excretion, in vitro digestion, manuka honey, PERFORMANCE LIQUID-CHROMATOGRAPHY, LEPTOSPERMUM-SCOPARIUM HONEY, D-LACTIC ACID, MANUKA HONEY, D-LACTATE, CARBONYL-COMPOUNDS, GLYOXALASE SYSTEM, ARGININE RESIDUES, DIABETIC-PATIENTS, MAILLARD REACTION