Identification of Pseudomonas asiatica subsp. bavariensis str. JM1 as the first Nε-carboxy(m)ethyllysine-degrading soil bacterium

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung


  • Judith Mehler - , Ludwig-Maximilians-Universität München (LMU) (Autor:in)
  • Kim Ina Behringer - , Technische Universität Dresden, Technische Universität Braunschweig (Autor:in)
  • Robert Ethan Rollins - , Ludwig-Maximilians-Universität München (LMU) (Autor:in)
  • Friederike Pisarz - , Johannes Gutenberg-Universität Mainz (Autor:in)
  • Andreas Klingl - , Ludwig-Maximilians-Universität München (LMU) (Autor:in)
  • Thomas Henle - , Professur für Lebensmittelchemie (LC1) (Autor:in)
  • Ralf Heermann - , Johannes Gutenberg-Universität Mainz (Autor:in)
  • Noémie S. Becker - , Ludwig-Maximilians-Universität München (LMU) (Autor:in)
  • Michael Hellwig - , Professur für Spezielle Lebensmittelchemie, Technische Universität Braunschweig (Autor:in)
  • Jürgen Lassak - , Ludwig-Maximilians-Universität München (LMU) (Autor:in)


Thermal food processing leads to the formation of advanced glycation end products (AGE) such as Nε-carboxymethyllysine (CML). Accordingly, these non-canonical amino acids are an important part of the human diet. However, CML is only partially decomposed by our gut microbiota and up to 30% are excreted via faeces and, hence, enter the environment. In frame of this study, we isolated a soil bacterium that can grow on CML as well as its higher homologue Nε-carboxyethyllysine (CEL) as sole source of carbon. Bioinformatic analyses upon whole-genome sequencing revealed a subspecies of Pseudomonas asiatica, which we named ‘bavariensis’. We performed a metabolite screening of P. asiatica subsp. bavariensis str. JM1 grown either on CML or CEL and identified N-carboxymethylaminopentanoic acid and N-carboxyethylaminopentanoic acid respectively. We further detected α-aminoadipate as intermediate in the metabolism of CML. These reaction products suggest two routes of degradation: While CEL seems to be predominantly processed from the α-C-atom, decomposition of CML can also be initiated with cleavage of the carboxymethyl group and under the release of acetate. Thus, our study provides novel insights into the metabolism of two important AGEs and how these are processed by environmental bacteria.


Seiten (von - bis)3229-3241
FachzeitschriftEnvironmental microbiology
PublikationsstatusVeröffentlicht - Juli 2022

Externe IDs

PubMed 35621031
ORCID /0000-0001-8528-6893/work/142256517



  • Bacteria/metabolism, Food Handling, Glycation End Products, Advanced/metabolism, Humans, Pseudomonas, Soil