Voltage-dependent ion channels in the mouse RPE: comparison with Norrie disease mice
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
We studied electrophysiological properties of cultured retinal pigment epithelial (RPE) cells from mouse and a mouse model for Norrie disease. Wild-type RPE cells revealed the expression of ion channels known from other species: delayed-rectifier K(+) channels composed of Kv1.3 subunits, inward rectifier K(+) channels, Ca(V)1.3 L-type Ca(2+) channels and outwardly rectifying Cl(-) channels. Expression pattern and the ion channel characteristics current density, blocker sensitivity, kinetics and voltage-dependence were compared in cells from wild-type and Norrie mice. Although no significant differences were observed, our study provides a base for future studies on ion channel function and dysfunction in transgenic mouse models.
Details
| Original language | English |
|---|---|
| Pages (from-to) | 688-98 |
| Number of pages | 11 |
| Journal | Vision Research |
| Volume | 46 |
| Issue number | 5 |
| Publication status | Published - Mar 2006 |
| Peer-reviewed | Yes |
External IDs
| Scopus | 29144487043 |
|---|---|
| ORCID | /0000-0002-0926-6556/work/150884376 |
Keywords
Keywords
- Animals, Blotting, Western, Calcium Channels, L-Type/metabolism, Cells, Cultured, Chloride Channels/metabolism, Disease Models, Animal, Eye Diseases, Hereditary/metabolism, Genetic Diseases, X-Linked/metabolism, Ion Channels/metabolism, Kv1.3 Potassium Channel/metabolism, Membrane Potentials, Mice, Mice, Knockout, Patch-Clamp Techniques, Pigment Epithelium of Eye/metabolism, Potassium Channels, Inwardly Rectifying/metabolism