Visualization of carious lesions with polarized and depolarized light microscopy

Research output: Contribution to journalResearch articleContributedpeer-review


Polarized light microscopy (PLM) is an established method in dental histology for investigating the ultrastructure and carious process of teeth. This study introduces a novel approach for measuring the degree of polarization (DOP) in a modified PLM setup and uses the DOP to assess the changes of the optical properties of enamel and dentin due to caries. The validation is provided by a comparison with complementary imaging methods, i.e. standard PLM and µCT. The results show that demineralization is reliably displayed by the DOP in accordance with the common imaging methods, and that this quantitative analysis of depolarization allows the characterization of the different pathohistological zones of caries.


Original languageEnglish
Pages (from-to)3018-3036
Number of pages19
JournalBiomedical optics express
Issue number5
Publication statusPublished - 1 May 2024

External IDs

ORCID /0000-0002-8160-3000/work/158306173
ORCID /0000-0003-0554-2178/work/158306231
ORCID /0009-0008-7642-8608/work/158306430
ORCID /0000-0003-2292-5533/work/158306452
Scopus 85192671781