Visualization of carious lesions with polarized and depolarized light microscopy

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Abstract

Polarized light microscopy (PLM) is an established method in dental histology for investigating the ultrastructure and carious process of teeth. This study introduces a novel approach for measuring the degree of polarization (DOP) in a modified PLM setup and uses the DOP to assess the changes of the optical properties of enamel and dentin due to caries. The validation is provided by a comparison with complementary imaging methods, i.e. standard PLM and µCT. The results show that demineralization is reliably displayed by the DOP in accordance with the common imaging methods, and that this quantitative analysis of depolarization allows the characterization of the different pathohistological zones of caries.

Details

OriginalspracheEnglisch
Seiten (von - bis)3018-3036
Seitenumfang19
FachzeitschriftBiomedical optics express
Jahrgang15
Ausgabenummer5
PublikationsstatusVeröffentlicht - 1 Mai 2024
Peer-Review-StatusJa

Externe IDs

ORCID /0000-0002-8160-3000/work/158306173
ORCID /0000-0003-0554-2178/work/158306231
ORCID /0009-0008-7642-8608/work/158306430
ORCID /0000-0003-2292-5533/work/158306452

Schlagworte