Verfahren zur Beschichtung eines Substrats mit einer Hydrophobinbilage und Substrat mit einer Hydrophobinbilagenbeschichtung
Research output: Intellectual property › Patent application/Patent
Contributors
- TUD Dresden University of Technology
Abstract
The method comprises providing a protein-containing coating solution, contacting a surface of the substrate with the protein-containing solution, removing a supernatant solution from the coated substrate, drying the coated substrate, separating protein units aggregated from an aqueous solution of self-assembling proteins for providing the coating solution, and generating monomers or oligomers of the self-assembling proteins by an addition of a solution of ionic surfactants and a salt-containing, alkaline and/or acidic solution to the protein-containing coating solution and then stabilizing. The method comprises providing a protein-containing coating solution, contacting a surface of the substrate with the protein-containing solution, removing a supernatant solution from the coated substrate, drying the coated substrate, separating protein units aggregated from an aqueous solution of self-assembling proteins for providing the coating solution, and generating monomers or oligomers of the self-assembling proteins by an addition of a solution of ionic surfactants, a salt-containing, alkaline and/or acidic solution to the protein-containing coating solution and then stabilizing, where the ionic surfactant is added so that only the surface-active part of each active protein monomer or predominant protein oligomer is enveloped by surfactant particles. Hydrophobins proteins, surface layer proteins and/or recombinant fusion proteins with two domains are used as self-assembling proteins, where the domain is a self-assembling protein domain and a functional domain. Independent claims are included for: (1) a protein-containing coating solution; and (2) a substrate with a protein-containing coating of self-assembling proteins.
| Translated title of the contribution | Coating substrate with monolayer of self-assembling proteins, by providing protein-containing coating solution, contacting surface of substrate with the protein-containing solution, and removing supernatant solution from coated substrate |
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Details
The method comprises providing a protein-containing coating solution, contacting a surface of the substrate with the protein-containing solution, removing a supernatant solution from the coated substrate, drying the coated substrate, separating protein units aggregated from an aqueous solution of self-assembling proteins for providing the coating solution, and generating monomers or oligomers of the self-assembling proteins by an addition of a solution of ionic surfactants and a salt-containing, alkaline and/or acidic solution to the protein-containing coating solution and then stabilizing. The method comprises providing a protein-containing coating solution, contacting a surface of the substrate with the protein-containing solution, removing a supernatant solution from the coated substrate, drying the coated substrate, separating protein units aggregated from an aqueous solution of self-assembling proteins for providing the coating solution, and generating monomers or oligomers of the self-assembling proteins by an addition of a solution of ionic surfactants, a salt-containing, alkaline and/or acidic solution to the protein-containing coating solution and then stabilizing, where the ionic surfactant is added so that only the surface-active part of each active protein monomer or predominant protein oligomer is enveloped by surfactant particles. Hydrophobins proteins, surface layer proteins and/or recombinant fusion proteins with two domains are used as self-assembling proteins, where the domain is a self-assembling protein domain and a functional domain. Independent claims are included for: (1) a protein-containing coating solution; and (2) a substrate with a protein-containing coating of self-assembling proteins.
| Original language | German |
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| IPC (International Patent Classification) | B05D 5/ 00 A I |
| Patent number | DE102011089241 |
| Country/Territory | Germany |
| Priority date | 20 Dec 2011 |
| Priority number | DE20111089241 |
| Publication status | Published - 11 Apr 2013 |