The Role of Polymorphisms at the Interleukin-1, Interleukin-4, GATA-3 and Cyclooxygenase-2 Genes in Non-Surgical Periodontal Therapy

Research output: Contribution to journalResearch articleContributedpeer-review


  • Kay Arne Walther - , Justus Liebig University Giessen (Author)
  • José Roberto Gonzales - , Justus Liebig University Giessen (Author)
  • Sabine Gröger - , Justus Liebig University Giessen (Author)
  • Benjamin Ehmke - , University of Münster (Author)
  • Dogan Kaner - , Charité – Universitätsmedizin Berlin, Witten/Herdecke University (Author)
  • Katrin Lorenz - , Department of Periodontology (Author)
  • Peter Eickholz - , University Hospital Frankfurt (Author)
  • Thomas Kocher - , University of Greifswald (Author)
  • Ti Sun Kim - , Heidelberg University  (Author)
  • Ulrich Schlagenhauf - , University of Würzburg (Author)
  • Raphael Koch - , University of Münster (Author)
  • Jörg Meyle - , Justus Liebig University Giessen (Author)


Periodontitis is a multifactorial disease. The aim of this explorative study was to investigate the role of Interleukin-(IL)-1, IL-4, GATA-3 and Cyclooxygenase-(COX)-2 polymorphisms after nonsurgical periodontal therapy with adjunctive systemic antibiotics (amoxicillin/metronidazole) and subsequent maintenance in a Caucasian population. Analyses were performed using blood samples from periodontitis patients of a multi-center trial ( NCT00707369=ABPARO-study). Polymorphisms were analyzed using quantitative real-time PCR. Clinical attachment levels (CAL), percentage of sites showing further attachment loss (PSAL) 1.3 mm, bleeding on probing (BOP) and plaque score were assessed. Exploratory statistical analysis was performed. A total of 209 samples were genotyped. Patients carrying heterozygous genotypes and single-nucleotide-polymorphisms (SNP) on the GATA-3-IVS4 +1468 gene locus showed less CAL loss than patients carrying wild type. Heterozygous genotypes and SNPs on the IL-1A-889, IL-1B +3954, IL-4-34, IL-4-590, GATA-3-IVS4 +1468 and COX-2-1195 gene loci did not influence CAL. In multivariate analysis, CAL was lower in patients carrying GATA-3 heterozygous genotypes and SNPs than those carrying wild-types. For the first time, effects of different genotypes were analyzed in periodontitis progression after periodontal therapy and during supportive treatment using systemic antibiotics demonstrating a slight association of GATA-3 gene locus with CAL. This result suggests that GATA-3 genotypes are a contributory but non-essential risk factor for periodontal disease progression.


Original languageEnglish
Article number7266
JournalInternational journal of molecular sciences
Issue number13
Publication statusPublished - 1 Jul 2022

External IDs

PubMed 35806269
ORCID /0000-0002-0228-6140/work/146167269



  • antibiotics, Cyclooxygenase-2, GATA-3, Interleukin-1, Interleukin-4, periodontal therapy, periodontitis, polymorphisms, risk factor