The molecular basis for pore pattern morphogenesis in diatom silica

Research output: Contribution to journalResearch articleContributedpeer-review

Abstract

Biomineral-forming organisms produce inorganic materials with complex, genetically encoded morphologies that are unmatched by current synthetic chemistry. It is poorly understood which genes are involved in biomineral morphogenesis and how the encoded proteins guide this process. We addressed these questions using diatoms, which are paradigms for the self-assembly of hierarchically meso- and macroporous silica under mild reaction conditions. Proteomics analysis of the intracellular organelle for silica biosynthesis led to the identification of new biomineralization proteins. Three of these, coined dAnk1-3, contain a common protein-protein interaction domain (ankyrin repeats), indicating a role in coordinating assembly of the silica biomineralization machinery. Knocking out individual dank genes led to aberrations in silica biogenesis that are consistent with liquid-liquid phase separation as underlying mechanism for pore pattern morphogenesis. Our work provides an unprecedented path for the synthesis of tailored mesoporous silica materials using synthetic biology.

Details

Original languageEnglish
Article numbere2211549119
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number49
Publication statusPublished - 2 Dec 2022
Peer-reviewedYes

External IDs

PubMed 36459651

Keywords

Research priority areas of TU Dresden

ASJC Scopus subject areas

Keywords

  • ankyrin-repeat domain, biomineralization, mesoporous silica, phase separation, silica deposition vesicle, Silicon Dioxide, Biomineralization, Ankyrin Repeat, Morphogenesis/genetics, Diatoms/genetics

Library keywords