The human kinesin-14 HSET tracks the tips of growing microtubules in vitro

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

Tip-tracking of kinesin-14 motor proteins is believed to be crucial for the assembly and maintenance of dynamic microtubule arrays. However, in contrast to other members of the kinesin-14 family, H. sapiens kinesin-14 HSET has so far never been observed to be prominently located at microtubule plus ends. Here, using an in vitro microtubule dynamics reconstitution assay we observe tip-tracking of GFP-HSET in the presence of H. sapiens EB1 (hsEB1). Tip-tracking depended on the SxIP-like motif in HSET as well as on the EB homology domain in hsEB1. D. melanogaster Ncd and S. pombe Klp2 tip-tracking reconstitution assays accompanied by kinesin-14 amino acid sequence comparisons suggest that SxIP-like motif mediated tip-tracking dependent on EB family proteins is conserved in the kinesin-14 family of molecular motors.

Details

Original languageEnglish
Pages (from-to)515-521
Number of pages7
JournalCytoskeleton
Volume70
Issue number9
Publication statusPublished - Sept 2013
Peer-reviewedYes

External IDs

Scopus 84885852166
ORCID /0000-0002-0750-8515/work/142235531

Keywords

Research priority areas of TU Dresden

Keywords

  • Amino Acid Sequence, Animals, Drosophila melanogaster, Humans, Kinesins/chemistry, Microscopy, Fluorescence, Microtubule-Associated Proteins/metabolism, Microtubules/chemistry, Molecular Sequence Data

Library keywords