The human kinesin-14 HSET tracks the tips of growing microtubules in vitro
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Tip-tracking of kinesin-14 motor proteins is believed to be crucial for the assembly and maintenance of dynamic microtubule arrays. However, in contrast to other members of the kinesin-14 family, H. sapiens kinesin-14 HSET has so far never been observed to be prominently located at microtubule plus ends. Here, using an in vitro microtubule dynamics reconstitution assay we observe tip-tracking of GFP-HSET in the presence of H. sapiens EB1 (hsEB1). Tip-tracking depended on the SxIP-like motif in HSET as well as on the EB homology domain in hsEB1. D. melanogaster Ncd and S. pombe Klp2 tip-tracking reconstitution assays accompanied by kinesin-14 amino acid sequence comparisons suggest that SxIP-like motif mediated tip-tracking dependent on EB family proteins is conserved in the kinesin-14 family of molecular motors.
Details
Original language | English |
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Pages (from-to) | 515-521 |
Number of pages | 7 |
Journal | Cytoskeleton |
Volume | 70 |
Issue number | 9 |
Publication status | Published - Sept 2013 |
Peer-reviewed | Yes |
External IDs
Scopus | 84885852166 |
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ORCID | /0000-0002-0750-8515/work/142235531 |
Keywords
Research priority areas of TU Dresden
Keywords
- Amino Acid Sequence, Animals, Drosophila melanogaster, Humans, Kinesins/chemistry, Microscopy, Fluorescence, Microtubule-Associated Proteins/metabolism, Microtubules/chemistry, Molecular Sequence Data