The human kinesin-14 HSET tracks the tips of growing microtubules in vitro
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Tip-tracking of kinesin-14 motor proteins is believed to be crucial for the assembly and maintenance of dynamic microtubule arrays. However, in contrast to other members of the kinesin-14 family, H. sapiens kinesin-14 HSET has so far never been observed to be prominently located at microtubule plus ends. Here, using an in vitro microtubule dynamics reconstitution assay we observe tip-tracking of GFP-HSET in the presence of H. sapiens EB1 (hsEB1). Tip-tracking depended on the SxIP-like motif in HSET as well as on the EB homology domain in hsEB1. D. melanogaster Ncd and S. pombe Klp2 tip-tracking reconstitution assays accompanied by kinesin-14 amino acid sequence comparisons suggest that SxIP-like motif mediated tip-tracking dependent on EB family proteins is conserved in the kinesin-14 family of molecular motors.
Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 515-521 |
| Seitenumfang | 7 |
| Fachzeitschrift | Cytoskeleton |
| Jahrgang | 70 |
| Ausgabenummer | 9 |
| Publikationsstatus | Veröffentlicht - Sept. 2013 |
| Peer-Review-Status | Ja |
Externe IDs
| Scopus | 84885852166 |
|---|---|
| ORCID | /0000-0002-0750-8515/work/142235531 |
Schlagworte
Forschungsprofillinien der TU Dresden
Schlagwörter
- Amino Acid Sequence, Animals, Drosophila melanogaster, Humans, Kinesins/chemistry, Microscopy, Fluorescence, Microtubule-Associated Proteins/metabolism, Microtubules/chemistry, Molecular Sequence Data