Staphylococcus aureus extracellular adherence protein serves as anti-inflammatory factor by inhibiting the recruitment of host leukocytes
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Staphylococcus aureus is a human pathogen that secretes proteins that contribute to bacterial colonization. Here we describe the extracellular adherence protein (Eap) as a novel anti-inflammatory factor that inhibits host leukocyte recruitment. Due to its direct interactions with the host adhesive proteins intercellular adhesion molecule 1 (ICAM-1), fibrinogen or vitronectin, Eap disrupted beta(2)-integrin and urokinase receptor mediated leukocyte adhesion in vitro. Whereas Eap-expressing S. aureus induced a 2 3-fold lower neutrophil recruitment in bacterial peritonitis in mice as compared with an Eap-negative strain, isolated Eap prevented beta(2)-integrin-dependent neutrophil recruitment in a mouse model of acute thioglycollate-induced peritonitis. Thus, the specific interactions with ICAM-1 and extracellular matrix proteins render Eap a potent anti-inflammatory factor, which may serve as a new therapeutic substance to block leukocyte extravasation in patients with hyperinflammatory pathologies.
Details
Original language | English |
---|---|
Pages (from-to) | 687-693 |
Number of pages | 7 |
Journal | Nature medicine |
Volume | 8 |
Issue number | 7 |
Publication status | Published - Jul 2002 |
Peer-reviewed | Yes |
Externally published | Yes |
External IDs
Scopus | 0036061581 |
---|
Keywords
Keywords
- Animals, Anti-Inflammatory Agents, Non-Steroidal/pharmacology, Bacterial Adhesion, Bacterial Proteins/pharmacology, Cell Adhesion/drug effects, Cell Line, Endothelium, Vascular/microbiology, Fibronectins/physiology, Humans, Intercellular Adhesion Molecule-1/physiology, Leukocytes/microbiology, Macrophage-1 Antigen/physiology, Mice, Mice, Inbred Strains, Peritonitis/microbiology, Staphylococcal Infections/microbiology, Staphylococcus aureus/physiology, U937 Cells, Umbilical Veins