Restoration of cone-circuit functionality in the regenerating adult zebrafish retina
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Unlike humans, teleosts like zebrafish exhibit robust retinal regeneration after injury from endogenous stem cells. However, it is unclear if regenerating cone photoreceptors regain physiological function and integrate correctly into post-synaptic circuits. We used two-photon calcium imaging of living adult retina to examine photoreceptor responses before and after light-induced lesions. To assess functional recovery of cones and downstream outer retinal circuits, we exploited color opponency; UV cones exhibit intrinsic Off-response to blue light, but On-response to green light, which depends on feedback signals from outer retinal circuits. Accordingly, we assessed the presence and quality of Off- vs. On-responses and found that regenerated UV cones regain both Off-responses to short-wavelength and On-responses to long-wavelength light within 3 months after lesion. Therefore, physiological circuit functionality is restored in regenerated cone photoreceptors, suggesting that inducing endogenous regeneration is a promising strategy for human retinal repair.
Details
Original language | English |
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Pages (from-to) | 2158-2170.e6 |
Journal | Developmental cell |
Volume | 59 |
Issue number | 16 |
Publication status | Published - 19 Aug 2024 |
Peer-reviewed | Yes |
External IDs
PubMed | 39096897 |
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ORCID | /0000-0002-7133-7474/work/166326015 |
Keywords
ASJC Scopus subject areas
Keywords
- blindness, Ca imaging, CNS, functional restoration, photoreceptors, physiological recovery, regeneration, retina, UV cones, zebrafish