Dielectrophoresis (DEP) enables continuous and label-free separation of (bio)microparticles with high sensitivity and selectivity, whereas the low throughput issue greatly confines its clinical application. Herein, we report a novel design of the DEP separator embedded with cylindrical interdigitated electrodes that incorporate hybrid floating electrode layout for (bio)microparticle separation at favorable throughput. To better predict microparticle trajectory in the scaled-up DEP platform, a theoretical model based on coupling of electrostatic, fluid and temperature fields is established, in which the effects of Joule heating-induced electrothermal and buoyancy flows on particles are considered. Size-based fractionation of polystyrene microspheres and dielectric properties-based isolation of MDA-MB-231 from blood cells are numerically realized, respectively, by the proposed separator with sample throughputs up to 2.6 mL/min. Notably, the induced flows can promote DEP discrimination of heterogeneous cells. This work provides a reference on tailoring design of enlarged DEP platforms for highly efficient separation of (bio)samples at high throughput.