Progression of osteogenic cell cultures grown on microtopographic titanium coated with calcium phosphate and functionalized with a type i collagen-derived peptide

Research output: Contribution to journalResearch articleContributedpeer-review


  • Karina K.Y. Pereira - , Universidade de São Paulo (Author)
  • Olívia C. Alves - , Universidade de São Paulo (Author)
  • Arthur B. Novaes - , Universidade de São Paulo (Author)
  • Fabíola S. De Oliveira - , Universidade de São Paulo (Author)
  • Ji Hyun Yi - , University of Montreal (Author)
  • Osvaldo Zaniquelli - , Universidade de São Paulo (Author)
  • Cornelia Wolf-Brandstetter - , Chair of Biomaterials, Institute of Materials Science (Author)
  • Dieter Scharnweber - , Chair of Biomaterials (Author)
  • Fabio Variola - , University of Ottawa (Author)
  • Antonio Nanci - , University of Montreal (Author)
  • Adalberto L. Rosa - , Universidade de São Paulo (Author)
  • Paulo T. De Oliveira - , Universidade de São Paulo (Author)


Background: The functionalization of metallic surfaces aims at promoting the cellular response at the biomaterial-tissue interface. This study investigates the effects of the functionalization of titanium (Ti) microtopography with a calcium phosphate (CaP) coating with and without peptide 15 (P-15), a synthetic peptide analog of the cell-binding domain of collagen I, on the in vitro progression of osteogenic cells. Methods: Sandblasting and acid etching (SBAE; control) Ti microtopography was coated with CaP, enabling the loading of two concentrations of P-15: 20 or 200 μg/mL. A machined Ti was also examined. Rat calvarial osteogenic cells were cultured on Ti disks with the surfaces mentioned above for periods up to 21 days (n = 180 per group). Results: CaP coating exhibited a submicron-scale needle-shaped structure. Although all surfaces were hydrophobic at time zero, functionalization increased hydrophilicity at equilibrium. Microtopographies exhibited a lower proportion of well-spread cells at 4 hours of culture and cells with long cytoplasmic extensions at day 3; modified SBAE supported higher cell viability and larger extracellular osteopontin (OPN) accumulation. For SBAE and modified SBAE, real-time polymerase chain reaction showed the following results: 1) lower levels for runt-related transcription factor 2 at 7 days and for bone sialoprotein at days 7 and 10 as well as higher OPN levels at days 7 and 10 compared to machined Ti; and 2) higher alkaline phosphatase levels at day 10 compared to day 7. At 14 and 21 days, modified SBAE supported higher proportions of red-dye-stained areas (calcium content). Conclusion: Addition of a CaP coating to SBAE Ti by itself may affect key events of in vitro osteogenesis, ultimately resulting in enhanced matrix mineralization; additional P-15 functionalization has only limited synergistic effects.


Original languageEnglish
Pages (from-to)1199-1210
Number of pages12
JournalJournal of periodontology
Issue number8
Publication statusPublished - Aug 2013

External IDs

Scopus 84882240771
PubMed 23088527
ORCID /0000-0001-9509-6145/work/145224969


ASJC Scopus subject areas


  • Calcium phosphates, Cells, cultured, Collagen, Osteoblasts, Peptides, Titanium