Precision Culture Scaling to Establish High-Throughput Vasculogenesis Models
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Hydrogel-based 3D cell cultures can recapitulate (patho)physiological phenomena ex vivo. However, due to their complex multifactorial regulation, adapting these tissue and disease models for high-throughput screening workflows remains challenging. In this study, a new precision culture scaling (PCS-X) methodology combines statistical techniques (design of experiment and multiple linear regression) with automated, parallelized experiments and analyses to customize hydrogel-based vasculogenesis cultures using human umbilical vein endothelial cells and retinal microvascular endothelial cells. Variations of cell density, growth factor supplementation, and media composition are systematically explored to induce vasculogenesis in endothelial mono- and cocultures with mesenchymal stromal cells or retinal microvascular pericytes in 384-well plate formats. The developed cultures are shown to respond to vasculogenesis inhibitors in a compound- and dose-dependent manner, demonstrating the scope and power of PCS-X in creating parallelized tissue and disease models for drug discovery and individualized therapies.
Details
Original language | English |
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Article number | 2400388 |
Journal | Advanced healthcare materials |
Volume | 13 |
Issue number | 18 |
Publication status | Published - 2024 |
Peer-reviewed | Yes |
External IDs
PubMed | 38465502 |
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Mendeley | 2ffc4085-d5ba-301a-8427-0b7ef169512b |
ORCID | /0000-0003-0189-3448/work/162347621 |
Keywords
Sustainable Development Goals
ASJC Scopus subject areas
Keywords
- cell-instructive hydrogels, design of experiments, high-throughput screening, in vitro tissue and disease models, multiple linear regression, vasculogenesis