Polyacrylamide gels containing ionized functional groups for the molecular imprinting of human growth hormone

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Rimantas Kublickas - (Author)
  • Carsten Werner - , Leibniz Institute of Polymer Research Dresden (Author)
  • Giedre Jariene - (Author)
  • Brigitte Voit - , Leibniz Institute of Polymer Research Dresden (Author)
  • Liudvikas Lasas - (Author)

Abstract

Networks of polyacrylamide having methacrylic acid and 2-(dimethylamino)ethyl methacrylate incorporated were studied for the possibility of imprinting human growth hormone. Two templates - the monomeric and the dimeric molecular forms of human growth hormone hormone - were for the first time imprinted using a hydrogel containing charged functional groups. The results show that approximately 90% (w/w) of the templates could be extracted from the molecularly imprinted acrylamide polymers. The molecularly imprinted polymer adsorbed approximately 70 times (for the monomeric form of the hormone) and 60 times (for the dimeric form) more of the template than of the non-imprinted polymer. The selectivity of the molecularly imprinted polymers was also studied.

Details

Original languageEnglish
Pages (from-to)611-617
Number of pages7
JournalPolymer bulletin
Volume58
Issue number3
Publication statusPublished - Mar 2007
Peer-reviewedYes
Externally publishedYes

External IDs

Scopus 33846609612
ORCID /0000-0002-4531-691X/work/148607744

Keywords

Keywords

  • Polymer particles, Recognition, Peptides, Lysozyme, Sites