On the enigma of glutathione-dependent styrene degradation in Gordonia rubripertincta CWB2
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Among bacteria, only a single styrene-specific degradation pathway has been reported so far. It comprises the activity of styrene monooxygenase, styrene oxide isomerase, and phenylacetaldehyde dehydrogenase, yielding phenylacetic acid as the central metabolite. The alternative route comprises ring-hydroxylating enzymes and yields vinyl catechol as central metabolite, which undergoes meta-cleavage. This was reported to be unspecific and also allows the degradation of benzene derivatives. However, some bacteria had been described to degrade styrene but do not employ one of those routes or only parts of them. Here, we describe a novel "hybrid" degradation pathway for styrene located on a plasmid of foreign origin. As putatively also unspecific, it allows metabolizing chemically analogous compounds (e.g., halogenated and/or alkylated styrene derivatives). Gordonia rubripertincta CWB2 was isolated with styrene as the sole source of carbon and energy. It employs an assembled route of the styrene side-chain degradation and isoprene degradation pathways that also funnels into phenylacetic acid as the central metabolite. Metabolites, enzyme activity, genome, transcriptome, and proteome data reinforce this observation and allow us to understand this biotechnologically relevant pathway, which can be used for the production of ibuprofen.
Details
Original language | English |
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Article number | e00154-18 |
Journal | Applied and environmental microbiology |
Volume | 84 |
Issue number | 9 |
Publication status | Published - 1 May 2018 |
Peer-reviewed | Yes |
Externally published | Yes |
External IDs
PubMed | 29475871 |
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ORCID | /0000-0002-7109-2788/work/142249497 |
Keywords
ASJC Scopus subject areas
Keywords
- Genomic island, Glutathione in actinobacteria, Glutathione S-transferase, Horizontal gene transfer, Hybrid gene cluster, Microbial ibuprofen production, Proteomics, Styrene monooxygenase, Transcriptomics, Xenobiotic compounds