Multi-parametric hydrogels support 3D invitro bioengineered microenvironment models of tumour angiogenesis
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Tumour microenvironment greatly influences the development and metastasis of cancer progression. The development of three dimensional (3D) culture models which mimic that displayed invivo can improve cancer biology studies and accelerate novel anticancer drug screening. Inspired by a systems biology approach, we have formed 3D invitro bioengineered tumour angiogenesis microenvironments within a glycosaminoglycan-based hydrogel culture system. This microenvironment model can routinely recreate breast and prostate tumour vascularisation. The multiple cell types cultured within this model were less sensitive to chemotherapy when compared with two dimensional (2D) cultures, and displayed comparative tumour regression to that displayed invivo. These features highlight the use of our invitro culture model as a complementary testing platform in conjunction with animal models, addressing key reduction and replacement goals of the future. We anticipate that this biomimetic model will provide a platform for the in-depth analysis of cancer development and the discovery of novel therapeutic targets.
Details
Original language | English |
---|---|
Pages (from-to) | 609-620 |
Number of pages | 12 |
Journal | Biomaterials |
Volume | 53 |
Publication status | Published - 1 Jun 2015 |
Peer-reviewed | Yes |
External IDs
PubMed | 25890757 |
---|---|
ORCID | /0000-0003-0189-3448/work/161890459 |
Keywords
ASJC Scopus subject areas
Keywords
- Angiogenesis, Carcinogenesis, Cell culture, ECM, Heparin, Hydrogel