Methods for Studying Appendicular Skeletal Biology in Axolotls

Research output: Contribution to book/conference proceedings/anthology/reportChapter in book/anthology/reportInvited

Abstract

The axolotl is a great model for studying cartilage, bone and joint regeneration, fracture healing, and evolution. Stainings such as Alcian Blue/Alizarin Red have become workhorses in skeletal analyses, but additional methods complement the detection of different skeletal matrices. Here we describe protocols for studying skeletal biology in axolotls, particularly Alcian Blue/Alizarin Red staining, microcomputed tomography (μCT) scan and live staining of calcified tissue. In addition, we describe a method for decalcification of skeletal elements to ease sectioning.

Details

Original languageEnglish
Title of host publicationSalamanders
EditorsAshley W. Seifert, Joshua D. Currie
PublisherHumana Press
Pages155-163
Number of pages9
Publication statusPublished - 23 Oct 2022
Peer-reviewedNo

Publication series

SeriesMethods in Molecular Biology (MIMB; Vol. 2562)
Number2562
ISSN1064-3745

External IDs

unpaywall 10.1007/978-1-0716-2659-7_9
Scopus 85140351048
Mendeley 554b7332-3637-3822-84be-284f2fcc1edf
ORCID /0000-0003-1802-5145/work/166764646

Keywords

Research priority areas of TU Dresden

DFG Classification of Subject Areas according to Review Boards

ASJC Scopus subject areas

Keywords

  • Animals, Alcian Blue, Ambystoma mexicanum, X-Ray Microtomography, Staining and Labeling, Biology, Calcification, Cartilage/bone, Skeleton, Live staining