The axolotl is a great model for studying cartilage, bone and joint regeneration, fracture healing, and evolution. Stainings such as Alcian Blue/Alizarin Red have become workhorses in skeletal analyses, but additional methods complement the detection of different skeletal matrices. Here we describe protocols for studying skeletal biology in axolotls, particularly Alcian Blue/Alizarin Red staining, microcomputed tomography (μCT) scan and live staining of calcified tissue. In addition, we describe a method for decalcification of skeletal elements to ease sectioning.
|Title of host publication||Methods in Molecular Biology|
|Number of pages||9|
|Publication status||Published - 2023|
|Series||Methods in molecular biology (Clifton, N.J.)|
Research priority areas of TU Dresden
- Animals, Alcian Blue, Ambystoma mexicanum, X-Ray Microtomography, Staining and Labeling, Biology, Calcification, Cartilage/bone, Skeleton, Live staining