Methods for Studying Appendicular Skeletal Biology in Axolotls
Research output: Contribution to book/Conference proceedings/Anthology/Report › Chapter in book/Anthology/Report › Invited
Contributors
Abstract
The axolotl is a great model for studying cartilage, bone and joint regeneration, fracture healing, and evolution. Stainings such as Alcian Blue/Alizarin Red have become workhorses in skeletal analyses, but additional methods complement the detection of different skeletal matrices. Here we describe protocols for studying skeletal biology in axolotls, particularly Alcian Blue/Alizarin Red staining, microcomputed tomography (μCT) scan and live staining of calcified tissue. In addition, we describe a method for decalcification of skeletal elements to ease sectioning.
Details
| Original language | English |
|---|---|
| Title of host publication | Salamanders |
| Editors | Ashley W. Seifert, Joshua D. Currie |
| Publisher | Humana Press |
| Pages | 155-163 |
| Number of pages | 9 |
| Publication status | Published - 23 Oct 2022 |
| Peer-reviewed | No |
Publication series
| Series | Methods in Molecular Biology (MIMB; Vol. 2562) |
|---|---|
| Number | 2562 |
| ISSN | 1064-3745 |
External IDs
| unpaywall | 10.1007/978-1-0716-2659-7_9 |
|---|---|
| Scopus | 85140351048 |
| Mendeley | 554b7332-3637-3822-84be-284f2fcc1edf |
| ORCID | /0000-0003-1802-5145/work/166764646 |
Keywords
Research priority areas of TU Dresden
DFG Classification of Subject Areas according to Review Boards
ASJC Scopus subject areas
Keywords
- Animals, Alcian Blue, Ambystoma mexicanum, X-Ray Microtomography, Staining and Labeling, Biology, Calcification, Cartilage/bone, Skeleton, Live staining