Methods for Studying Appendicular Skeletal Biology in Axolotls

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Abstract

The axolotl is a great model for studying cartilage, bone and joint regeneration, fracture healing, and evolution. Stainings such as Alcian Blue/Alizarin Red have become workhorses in skeletal analyses, but additional methods complement the detection of different skeletal matrices. Here we describe protocols for studying skeletal biology in axolotls, particularly Alcian Blue/Alizarin Red staining, microcomputed tomography (μCT) scan and live staining of calcified tissue. In addition, we describe a method for decalcification of skeletal elements to ease sectioning.

Details

OriginalspracheEnglisch
TitelSalamanders
Redakteure/-innenAshley W. Seifert, Joshua D. Currie
Herausgeber (Verlag)Humana Press
Seiten155-163
Seitenumfang9
PublikationsstatusVeröffentlicht - 23 Okt. 2022
Peer-Review-StatusNein

Publikationsreihe

ReiheMethods in Molecular Biology (MIMB; Vol. 2562)
Nummer2562
ISSN1064-3745

Externe IDs

unpaywall 10.1007/978-1-0716-2659-7_9
Scopus 85140351048
Mendeley 554b7332-3637-3822-84be-284f2fcc1edf
ORCID /0000-0003-1802-5145/work/166764646

Schlagworte

Forschungsprofillinien der TU Dresden

DFG-Fachsystematik nach Fachkollegium

ASJC Scopus Sachgebiete

Schlagwörter

  • Animals, Alcian Blue, Ambystoma mexicanum, X-Ray Microtomography, Staining and Labeling, Biology, Calcification, Cartilage/bone, Skeleton, Live staining