Methods for Studying Appendicular Skeletal Biology in Axolotls
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Beitragende
Abstract
The axolotl is a great model for studying cartilage, bone and joint regeneration, fracture healing, and evolution. Stainings such as Alcian Blue/Alizarin Red have become workhorses in skeletal analyses, but additional methods complement the detection of different skeletal matrices. Here we describe protocols for studying skeletal biology in axolotls, particularly Alcian Blue/Alizarin Red staining, microcomputed tomography (μCT) scan and live staining of calcified tissue. In addition, we describe a method for decalcification of skeletal elements to ease sectioning.
Details
Originalsprache | Englisch |
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Titel | Salamanders |
Redakteure/-innen | Ashley W. Seifert, Joshua D. Currie |
Herausgeber (Verlag) | Humana Press |
Seiten | 155-163 |
Seitenumfang | 9 |
Publikationsstatus | Veröffentlicht - 23 Okt. 2022 |
Peer-Review-Status | Nein |
Publikationsreihe
Reihe | Methods in Molecular Biology (MIMB; Vol. 2562) |
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Nummer | 2562 |
ISSN | 1064-3745 |
Externe IDs
unpaywall | 10.1007/978-1-0716-2659-7_9 |
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Scopus | 85140351048 |
Mendeley | 554b7332-3637-3822-84be-284f2fcc1edf |
ORCID | /0000-0003-1802-5145/work/166764646 |
Schlagworte
Forschungsprofillinien der TU Dresden
DFG-Fachsystematik nach Fachkollegium
ASJC Scopus Sachgebiete
Schlagwörter
- Animals, Alcian Blue, Ambystoma mexicanum, X-Ray Microtomography, Staining and Labeling, Biology, Calcification, Cartilage/bone, Skeleton, Live staining