Metabolic alterations of Verbascum nigrum L. plants and SAArT transformed roots as revealed by NMR-based metabolomics

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Milen I. Georgiev - , Bulgarian Academy of Sciences (Author)
  • Anna Radziszewska - , TUD Dresden University of Technology (Author)
  • Madeleine Neumann - , Institute of Botany (Author)
  • Andrey Marchev - , Bulgarian Academy of Sciences (Author)
  • Kalina Alipieva - , Bulgarian Academy of Sciences (Author)
  • Jutta Ludwig-Müller - , Institute of Botany (Author)

Abstract

Verbascum nigrum L. (dark mullein) is known to be a rich source of bioactive secondary metabolites, including high value iridoid and phenylethanoid glycosides, hence the development of alternative approaches for their sustainable supply is highly relevant. Here we report the induction of a transformed root culture of V. nigrum by applying sonication assisted Agrobacterium rhizogenes-mediated genetic transformation. Further, nuclear magnetic resonance (NMR)-based metabolomics approach has been successfully applied to study metabolic differences of the V. nigrum mother plant and related transformed roots. 1H NMR fingerprinting in combination with 2D NMR and multivariate data analysis revealed that the most abundant molecule in the hairy root culture is glutamine, which is not found in the mother plant tissue. These findings provide important insights on the metabolic alterations as a result of the transformation of the host plant genome and development of so-called hairy roots.

Details

Original languageEnglish
Pages (from-to)349-356
Number of pages8
JournalPlant cell, tissue and organ culture : an international journal on biotechnology of higher plants
Volume123
Issue number2
Publication statusPublished - 1 Nov 2015
Peer-reviewedYes

Keywords

ASJC Scopus subject areas

Keywords

  • Agrobacterium rhizogenes, Dark mullein, Glutamine, Hairy roots, HSQC

Library keywords