Irradiation affects cellular properties and Eph receptor expression in human melanoma cells

Research output: Contribution to journalResearch articleContributedpeer-review


  • Birgit Mosch - , Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Doreen Pietzsch - , Carus Center for Teaching Excellence (CarL), Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Jens Pietzsch - , Helmholtz-Zentrum Dresden-Rossendorf (Author)


X-ray irradiation influences metastatic properties of tumor cells and, moreover, metastasis and cellular motility can be modified by members of the Eph receptor/ephrin family of receptor tyrosine kinases. We hypothesized that irradiation-induced changes in cellular properties relevant for metastasis in melanoma cells could be mediated by Eph receptor/ephrin signaling. In this pilot study, we analyzed one pre-metastatic (Mel-Juso) and three metastatic human melanoma (Mel-Juso-L3, A375, and A2058) cells lines and predominantly found anti-metastatic effects of X-ray irradiation with impaired cell growth, clonal growth and motility. Additionally, we observed an irradiation-induced increase in adhesion paralleled by a decrease in migration in Mel-Juso and Mel-Juso-L3 cells and, in part, also in A375 cells. We further demonstrate a decrease of EphA2 both in expression and activity at 7 d after irradiation paralleled by an upregulation of EphA3. Analyzing downstream signaling after irradiation, we detected decreased Src kinase phosphorylation, but unchanged focal adhesion kinase (FAK) phosphorylation, indicating, in part, irradiation-induced downregulation of signaling via the EphA2-Src-FAK axis in melanoma cells. However, to which extent this finding contributes to the modification of metastasis-relevant cellular properties remains to be elucidated.


Original languageEnglish
Pages (from-to)113-125
Number of pages13
JournalCell adhesion & migration
Issue number2
Publication statusPublished - 2012

External IDs

PubMed 22568947


Sustainable Development Goals


  • Eph receptors, Ephrins, Malignant skin cancer, Metastasis, Radiation therapy

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