In Vivo Analysis of Pathways Regulating Epithelial Polarity and Secretion Using Drosophila Salivary Glands

Research output: Contribution to journalResearch articleContributedpeer-review



The experimental versatility of the fruit fly has helped to uncover the molecular basis of epithelial cell polarity. In this chapter, we provide protocols to dissect Drosophila larval salivary glands (SGs) for ex vivo culture and live imaging, and for fixing and immunostaining for analysis by fluorescence microscopy. We describe how to combine these approaches with genetic and pharmacological assays. These techniques can be applied to study signaling pathways regulating epithelial cell polarity, membrane trafficking, gland secretion, and their impacts on animal feeding behavior.


Original languageEnglish
Pages (from-to)323-344
Number of pages22
JournalMethods in Molecular Biology
Publication statusPublished - 2022

External IDs

PubMed 35147952
unpaywall 10.1007/978-1-0716-2035-9_21



  • Apical membrane, Epithelial polarity, Intracellular trafficking, Live imaging, PIP2, Salivary glands, Secretion, Cell Polarity, Salivary Glands, Drosophila Proteins/genetics, Larva/metabolism, Drosophila melanogaster/genetics, Animals, Drosophila/metabolism