In Vivo Analysis of Pathways Regulating Epithelial Polarity and Secretion Using Drosophila Salivary Glands
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
The experimental versatility of the fruit fly has helped to uncover the molecular basis of epithelial cell polarity. In this chapter, we provide protocols to dissect Drosophila larval salivary glands (SGs) for ex vivo culture and live imaging, and for fixing and immunostaining for analysis by fluorescence microscopy. We describe how to combine these approaches with genetic and pharmacological assays. These techniques can be applied to study signaling pathways regulating epithelial cell polarity, membrane trafficking, gland secretion, and their impacts on animal feeding behavior.
Details
Originalsprache | Englisch |
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Seiten (von - bis) | 323-344 |
Seitenumfang | 22 |
Fachzeitschrift | Methods in Molecular Biology |
Jahrgang | 2438 |
Publikationsstatus | Veröffentlicht - 2022 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 35147952 |
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unpaywall | 10.1007/978-1-0716-2035-9_21 |
Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- Apical membrane, Epithelial polarity, Intracellular trafficking, Live imaging, PIP2, Salivary glands, Secretion, Cell Polarity, Salivary Glands, Drosophila Proteins/genetics, Larva/metabolism, Drosophila melanogaster/genetics, Animals, Drosophila/metabolism