In Vivo Analysis of Pathways Regulating Epithelial Polarity and Secretion Using Drosophila Salivary Glands

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

Abstract

The experimental versatility of the fruit fly has helped to uncover the molecular basis of epithelial cell polarity. In this chapter, we provide protocols to dissect Drosophila larval salivary glands (SGs) for ex vivo culture and live imaging, and for fixing and immunostaining for analysis by fluorescence microscopy. We describe how to combine these approaches with genetic and pharmacological assays. These techniques can be applied to study signaling pathways regulating epithelial cell polarity, membrane trafficking, gland secretion, and their impacts on animal feeding behavior.

Details

OriginalspracheEnglisch
Seiten (von - bis)323-344
Seitenumfang22
FachzeitschriftMethods in Molecular Biology
Jahrgang2438
PublikationsstatusVeröffentlicht - 2022
Peer-Review-StatusJa

Externe IDs

PubMed 35147952
unpaywall 10.1007/978-1-0716-2035-9_21

Schlagworte

Schlagwörter

  • Apical membrane, Epithelial polarity, Intracellular trafficking, Live imaging, PIP2, Salivary glands, Secretion, Cell Polarity, Salivary Glands, Drosophila Proteins/genetics, Larva/metabolism, Drosophila melanogaster/genetics, Animals, Drosophila/metabolism