Impedimetric Nanobiosensor for the Detection of SARS-CoV-2 Antigens and Antibodies

Research output: Contribution to journalResearch articleContributedpeer-review


  • Diana Isabel Sandoval Bojórquez - , Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Željko Janićijević - , Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Brenda Palestina Romero - , Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Eduardo Sergio Oliveros Mata - , Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Markus Laube - , Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Anja Feldmann - , Helmholtz-Zentrum Dresden-Rossendorf, University Cancer Center (UCC) (Author)
  • Alexandra Kegler - , Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Laura Drewitz - , Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Ciarán Fowley - , Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Jens Pietzsch - , Helmholtz-Zentrum Dresden-Rossendorf, TUD Dresden University of Technology (Author)
  • Juergen Fassbender - , Chair of Applied Solid State Physics, Helmholtz-Zentrum Dresden-Rossendorf (Author)
  • Torsten Tonn - , Division Transfusion Medicine, University Medicine (Faculty of Medicine and University Hospital), Department of internal Medicine I (Author)
  • Michael Bachmann - , University Cancer Centre, Helmholtz-Zentrum Dresden-Rossendorf, University Hospital Carl Gustav Carus Dresden, German Cancer Research Center (DKFZ) (Author)
  • Larysa Baraban - , Chair of Materials Science and Nanotechnology, Helmholtz-Zentrum Dresden-Rossendorf (Author)


Detection of antigens and antibodies (Abs) is of great importance in determining the infection and immunity status of the population, as they are key parameters guiding the handling of pandemics. Current point-of-care (POC) devices are a convenient option for rapid screening; however, their sensitivity requires further improvement. We present an interdigitated gold nanowire-based impedance nanobiosensor to detect COVID-19-associated antigens (receptor-binding domain of S1 protein of the SARS-CoV-2 virus) and respective Abs appearing during and after infection. The electrochemical impedance spectroscopy technique was used to assess the changes in measured impedance resulting from the binding of respective analytes to the surface of the chip. After 20 min of incubation, the sensor devices demonstrate a high sensitivity of about 57 pS·sn per concentration decade and a limit of detection (LOD) of 0.99 pg/mL for anti-SARS-CoV-2 Abs and a sensitivity of around 21 pS·sn per concentration decade and an LOD of 0.14 pg/mL for the virus antigen detection. Finally, the analysis of clinical plasma samples demonstrates the applicability of the developed platform to assist clinicians and authorities in determining the infection or immunity status of the patients.


Original languageEnglish
Pages (from-to)576-586
Number of pages11
JournalACS sensors
Issue number2
Publication statusPublished - 24 Feb 2023

External IDs

PubMed 36763494



  • SARS-CoV-2, electrochemical impedance spectroscopy (EIS), gold nanowires, impedance model, impedimetric sensing, point-of-care testing, reliability, surface plasmon resonance (SPR), Limit of Detection, Humans, Antibodies, Viral, COVID-19, Point-of-Care Systems