Identification and expression patterns of novel long non-coding RNAs in neural progenitors of the developing mammalian cortex

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

Long non-coding (lnc)RNAs play key roles in many biological processes. Elucidating the function of lncRNAs in cell type specification during organ development requires knowledge about their expression in individual progenitor types rather than in whole tissues. To achieve this during cortical development, we used a dual-reporter mouse line to isolate coexisting proliferating neural stem cells, differentiating neurogenic progenitors and newborn neurons and assessed the expression of lncRNAs by paired-end, high-throughput sequencing. We identified 379 genomic loci encoding novel lncRNAs and performed a comprehensive assessment of cell-specific expression patterns for all, annotated and novel, lncRNAs described to date. Our study provides a powerful new resource for studying these elusive transcripts during stem cell commitment and neurogenesis.

Details

Original languageEnglish
Article numbere995524
Number of pages9
JournalNeurogenesis
Volume2
Issue number1
Publication statusPublished - 2015
Peer-reviewedYes

External IDs

Scopus 84926144723
ORCID /0000-0002-3274-7163/work/142249709
ORCID /0000-0002-8749-7878/work/142251295

Keywords

Keywords

  • Cortical development, lncRNAs, Neurogenesis