Four-color fluorescence correlation spectroscopy realized in a grating-based detection platform

Research output: Contribution to journalResearch articleContributedpeer-review


  • Markus Burkhardt - , TUD Dresden University of Technology (Author)
  • Katrin G. Heinze - , TUD Dresden University of Technology (Author)
  • Petra Schwille - , Chair of Biophysics (Author)


We have developed a filterless multicolor detection unit for fluorescence correlation spectroscopy (FCS). This grating-based setup is continuously tunable for multicolor separation and is thus a powerful alternative to the classical cascade of dichroic mirrors and filters. Our tailored platform allows for accommodation of up to 15 detection channels covering the entire visible spectral range. As a proof of principle, we successfully demonstrate simultaneous FCS of four distinct fluorescent quantum dot species being mixed in aqueous solution. Grating-based detection allows for spectral high-resolution FCS in a stable and compact setup and is a feasible tool for quantitative investigation of complex biomolecular dynamics on a single molecule level.


Original languageEnglish
Pages (from-to)2266-2268
Number of pages3
JournalOptics letters
Issue number17
Publication statusPublished - 1 Sept 2005

External IDs

PubMed 16190439


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