Four-color fluorescence correlation spectroscopy realized in a grating-based detection platform

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Markus Burkhardt - , TUD Dresden University of Technology (Author)
  • Katrin G. Heinze - , TUD Dresden University of Technology (Author)
  • Petra Schwille - , Chair of Biophysics (Author)

Abstract

We have developed a filterless multicolor detection unit for fluorescence correlation spectroscopy (FCS). This grating-based setup is continuously tunable for multicolor separation and is thus a powerful alternative to the classical cascade of dichroic mirrors and filters. Our tailored platform allows for accommodation of up to 15 detection channels covering the entire visible spectral range. As a proof of principle, we successfully demonstrate simultaneous FCS of four distinct fluorescent quantum dot species being mixed in aqueous solution. Grating-based detection allows for spectral high-resolution FCS in a stable and compact setup and is a feasible tool for quantitative investigation of complex biomolecular dynamics on a single molecule level.

Details

Original languageEnglish
Pages (from-to)2266-2268
Number of pages3
JournalOptics letters
Volume30
Issue number17
Publication statusPublished - 1 Sept 2005
Peer-reviewedYes

External IDs

PubMed 16190439

Keywords

Library keywords