Four-color fluorescence correlation spectroscopy realized in a grating-based detection platform
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
We have developed a filterless multicolor detection unit for fluorescence correlation spectroscopy (FCS). This grating-based setup is continuously tunable for multicolor separation and is thus a powerful alternative to the classical cascade of dichroic mirrors and filters. Our tailored platform allows for accommodation of up to 15 detection channels covering the entire visible spectral range. As a proof of principle, we successfully demonstrate simultaneous FCS of four distinct fluorescent quantum dot species being mixed in aqueous solution. Grating-based detection allows for spectral high-resolution FCS in a stable and compact setup and is a feasible tool for quantitative investigation of complex biomolecular dynamics on a single molecule level.
Details
Original language | English |
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Pages (from-to) | 2266-2268 |
Number of pages | 3 |
Journal | Optics letters |
Volume | 30 |
Issue number | 17 |
Publication status | Published - 1 Sept 2005 |
Peer-reviewed | Yes |
External IDs
PubMed | 16190439 |
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