Four-color fluorescence correlation spectroscopy realized in a grating-based detection platform

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Markus Burkhardt - , Technische Universität Dresden (Autor:in)
  • Katrin G. Heinze - , Technische Universität Dresden (Autor:in)
  • Petra Schwille - , Professur für Biophysik (Autor:in)

Abstract

We have developed a filterless multicolor detection unit for fluorescence correlation spectroscopy (FCS). This grating-based setup is continuously tunable for multicolor separation and is thus a powerful alternative to the classical cascade of dichroic mirrors and filters. Our tailored platform allows for accommodation of up to 15 detection channels covering the entire visible spectral range. As a proof of principle, we successfully demonstrate simultaneous FCS of four distinct fluorescent quantum dot species being mixed in aqueous solution. Grating-based detection allows for spectral high-resolution FCS in a stable and compact setup and is a feasible tool for quantitative investigation of complex biomolecular dynamics on a single molecule level.

Details

OriginalspracheEnglisch
Seiten (von - bis)2266-2268
Seitenumfang3
FachzeitschriftOptics letters
Jahrgang30
Ausgabenummer17
PublikationsstatusVeröffentlicht - 1 Sept. 2005
Peer-Review-StatusJa

Externe IDs

PubMed 16190439

Schlagworte

Bibliotheksschlagworte