Fluorescence Imaging of Single Kinesin Motors on Immobilized Microtubules
Research output: Contribution to book/conference proceedings/anthology/report › Chapter in book/anthology/report › Contributed › peer-review
Contributors
Abstract
Recent developments in optical microscopy and nanometer tracking have greatly improved our understanding of cytoskeletal motor proteins. Using fluorescence microscopy, dynamic interactions are now routinely observed in vitro on the level of single molecules mainly using a geometry, where fluorescently labeled motors move on surface-immobilized filaments. In this chapter, we review recent methods related to single-molecule kinesin motility assays. In particular, we aim to provide practical advice on: how to set up the assays, how to acquire high-precision data from fluorescently labeled kinesin motors and attached quantum dots, and how to analyze data by nanometer tracking.
Details
Original language | English |
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Title of host publication | Single Molecule Analysis |
Publisher | Humana Press |
Pages | 121-137 |
Number of pages | 17 |
ISBN (print) | 9781617792816 |
Publication status | Published - 2011 |
Peer-reviewed | Yes |
Externally published | Yes |
Publication series
Series | Methods in Molecular Biology |
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Volume | 783 |
ISSN | 1064-3745 |
External IDs
PubMed | 21909886 |
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ORCID | /0000-0002-0750-8515/work/161407040 |
Keywords
ASJC Scopus subject areas
Keywords
- Imaging, Kinesin, Microtubule, Motor protein, Single molecule, TIRF microscopy