Fluorescence Imaging of Single Kinesin Motors on Immobilized Microtubules
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Beitragende
Abstract
Recent developments in optical microscopy and nanometer tracking have greatly improved our understanding of cytoskeletal motor proteins. Using fluorescence microscopy, dynamic interactions are now routinely observed in vitro on the level of single molecules mainly using a geometry, where fluorescently labeled motors move on surface-immobilized filaments. In this chapter, we review recent methods related to single-molecule kinesin motility assays. In particular, we aim to provide practical advice on: how to set up the assays, how to acquire high-precision data from fluorescently labeled kinesin motors and attached quantum dots, and how to analyze data by nanometer tracking.
Details
Originalsprache | Englisch |
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Titel | Single Molecule Analysis |
Herausgeber (Verlag) | Humana Press |
Seiten | 121-137 |
Seitenumfang | 17 |
ISBN (Print) | 9781617792816 |
Publikationsstatus | Veröffentlicht - 2011 |
Peer-Review-Status | Ja |
Extern publiziert | Ja |
Publikationsreihe
Reihe | Methods in Molecular Biology |
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Band | 783 |
ISSN | 1064-3745 |
Externe IDs
PubMed | 21909886 |
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ORCID | /0000-0002-0750-8515/work/161407040 |
Schlagworte
ASJC Scopus Sachgebiete
Schlagwörter
- Imaging, Kinesin, Microtubule, Motor protein, Single molecule, TIRF microscopy