Fluorescence correlation spectroscopy to examine protein-lipid interactions in membranes

Research output: Contribution to book/Conference proceedings/Anthology/ReportChapter in book/Anthology/ReportContributedpeer-review

Contributors

Abstract

Fluorescence correlation spectroscopy (FCS) is a versatile technique to study membrane dynamics and protein-lipid interactions. It can provide information about diffusion coefficients, concentrations, and molecular interactions of proteins and lipids in the membrane. These parameters allow the determination of protein partitioning into different lipid environments, the identification of lipid domains, and the detection of lipid-protein complexes on the membrane. During the last decade, FCS studies were successfully performed on model membrane systems as also on living cells, to characterize protein-lipid interactions. Recent developments of the method described here improved quantitative measurements on membranes and decreased the number of potential artifacts. The aim of this chapter is to provide the reader with the necessary information and some practical guidelines to perform FCS studies on artificial and cellular membranes.

Details

Original languageEnglish
Title of host publicationLipid-Protein Interactions
PublisherHumana Press
Pages253-278
Number of pages26
ISBN (print)9781627032742
Publication statusPublished - 2013
Peer-reviewedYes

Publication series

SeriesMethods in Molecular Biology
Volume974
ISSN1064-3745

External IDs

PubMed 23404280

Keywords

ASJC Scopus subject areas

Keywords

  • Cross correlation, Diffusion, FCS, Fluorescence correlation spectroscopy, Giant unilamellar vesicles, Lipid rafts, Model membranes, Protein-lipid interactions