Fluorescence correlation spectroscopy to examine protein-lipid interactions in membranes

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Beitragende

Abstract

Fluorescence correlation spectroscopy (FCS) is a versatile technique to study membrane dynamics and protein-lipid interactions. It can provide information about diffusion coefficients, concentrations, and molecular interactions of proteins and lipids in the membrane. These parameters allow the determination of protein partitioning into different lipid environments, the identification of lipid domains, and the detection of lipid-protein complexes on the membrane. During the last decade, FCS studies were successfully performed on model membrane systems as also on living cells, to characterize protein-lipid interactions. Recent developments of the method described here improved quantitative measurements on membranes and decreased the number of potential artifacts. The aim of this chapter is to provide the reader with the necessary information and some practical guidelines to perform FCS studies on artificial and cellular membranes.

Details

OriginalspracheEnglisch
TitelLipid-Protein Interactions
Herausgeber (Verlag)Humana Press
Seiten253-278
Seitenumfang26
ISBN (Print)9781627032742
PublikationsstatusVeröffentlicht - 2013
Peer-Review-StatusJa

Publikationsreihe

ReiheMethods in Molecular Biology
Band974
ISSN1064-3745

Externe IDs

PubMed 23404280

Schlagworte

ASJC Scopus Sachgebiete

Schlagwörter

  • Cross correlation, Diffusion, FCS, Fluorescence correlation spectroscopy, Giant unilamellar vesicles, Lipid rafts, Model membranes, Protein-lipid interactions