Exploitation of Engineered Light-Switchable Myosin XI for Nanotechnological Applications

Research output: Contribution to journalResearch articleContributedpeer-review


  • Aseem Salhotra - , Linnaeus University (Author)
  • Mohammad A. Rahman - , Linnaeus University (Author)
  • Paul V. Ruijgrok - , Stanford Engineering (Author)
  • Christoph R. Meinecke - , Chemnitz University of Technology (Author)
  • Marko Ušaj - , Linnaeus University (Author)
  • Sasha Zemsky - , Stanford Engineering (Author)
  • Frida W. Lindberg - , Lund University (Author)
  • Pradheebha Surendiran - , Lund University (Author)
  • Roman W. Lyttleton - , Lund University (Author)
  • Heiner Linke - , Lund University (Author)
  • Till Korten - , Clusters of Excellence PoL: Physics of Life, Core Facility Tailored Smart Microscopy, B CUBE Center for Molecular and Cellular Bioengineering (Author)
  • Zev Bryant - , Stanford Engineering (Author)
  • Alf Månsson - , Linnaeus University (Author)


For certain nanotechnological applications of the contractile proteins actin and myosin, e.g., in biosensing and network-based biocomputation, it would be desirable to temporarily switch on/off motile function in parts of nanostructured devices, e.g., for sorting or programming. Myosin XI motor constructs, engineered with a light-switchable domain for switching actin motility between high and low velocities (light-sensitive motors (LSMs) below), are promising in this regard. However, they were not designed for use in nanotechnology, where longevity of operation, long shelf life, and selectivity of function in specific regions of a nanofabricated network are important. Here, we tested if these criteria can be fulfilled using existing LSM constructs or if additional developments will be required. We demonstrated extended shelf life as well as longevity of the actin-propelling function compared to those in previous studies. We also evaluated several approaches for selective immobilization with a maintained actin propelling function in dedicated nanochannels only. Whereas selectivity was feasible using certain nanopatterning combinations, the reproducibility was not satisfactory. In summary, the study demonstrates the feasibility of using engineered light-controlled myosin XI motors for myosin-driven actin transport in nanotechnological applications. Before use for, e.g., sorting or programming, additional work is however needed to achieve reproducibility of the nanofabrication and, further, optimize the motor properties.


Original languageEnglish
Pages (from-to)17233-17244
Number of pages12
JournalACS nano
Issue number17
Publication statusPublished - 12 Sept 2023

External IDs

PubMed 37639711



  • actin, engineered myosin XI, light-switchable motor, nanofabrication, spatiotemporal motility control, surface chemistry