Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater
Research output: Contribution to journal › Research article › Contributed › peer-review
Contributors
Abstract
Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcription quantitative PCR (E- and S-gene) and droplet digital PCR. The concentration of virus with precipitation resulted in mean recoveries between 59.4% and 63.7% whereas rates from 33.0% to 42.6% after ultrafiltration of samples were demonstrated. The results suggest that the use of both methods allows an effective and practicable enrichment of SARS-CoV-2 from raw wastewater.
Details
Original language | English |
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Article number | 195 |
Number of pages | 7 |
Journal | Pathogens |
Volume | 10 |
Issue number | 2 |
Publication status | Published - 12 Feb 2021 |
Peer-reviewed | Yes |
External IDs
PubMed | 33673032 |
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PubMedCentral | PMC7917696 |
ORCID | /0000-0003-4963-7523/work/142242900 |
ORCID | /0000-0003-1526-997X/work/142247236 |
ORCID | /0000-0002-9301-1803/work/161409763 |
Keywords
Sustainable Development Goals
ASJC Scopus subject areas
Keywords
- Monitoring, SARS-CoV-2, Surveillance, Virus concentration, Wastewater, Wastewater-based epidemiology