Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

Abstract

Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcription quantitative PCR (E- and S-gene) and droplet digital PCR. The concentration of virus with precipitation resulted in mean recoveries between 59.4% and 63.7% whereas rates from 33.0% to 42.6% after ultrafiltration of samples were demonstrated. The results suggest that the use of both methods allows an effective and practicable enrichment of SARS-CoV-2 from raw wastewater.

Details

Original languageEnglish
Article number195
Number of pages7
JournalPathogens
Volume10
Issue number2
Publication statusPublished - 12 Feb 2021
Peer-reviewedYes

External IDs

PubMed 33673032
PubMedCentral PMC7917696
ORCID /0000-0003-4963-7523/work/142242900
ORCID /0000-0003-1526-997X/work/142247236
ORCID /0000-0002-9301-1803/work/161409763

Keywords

Sustainable Development Goals

Keywords

  • Monitoring, SARS-CoV-2, Surveillance, Virus concentration, Wastewater, Wastewater-based epidemiology

Library keywords