Evaluation of Two Methods to Concentrate SARS-CoV-2 from Untreated Wastewater
Publikation: Beitrag in Fachzeitschrift › Forschungsartikel › Beigetragen › Begutachtung
Beitragende
Abstract
Use of wastewater-based epidemiology as a tool to record and manage the course of SARS-CoV-2 infections in human populations requires information about the efficiency of methods to concentrate the virus from wastewater. In the present study, we spiked untreated wastewater with quantified SARS-CoV-2 positive clinical material and enriched the virus by polyethylene glycol precipitation and ultrafiltration with Vivaspin 10 kDa MWCO columns. SARS-CoV-2 was detected and quantified by reverse transcription quantitative PCR (E- and S-gene) and droplet digital PCR. The concentration of virus with precipitation resulted in mean recoveries between 59.4% and 63.7% whereas rates from 33.0% to 42.6% after ultrafiltration of samples were demonstrated. The results suggest that the use of both methods allows an effective and practicable enrichment of SARS-CoV-2 from raw wastewater.
Details
Originalsprache | Englisch |
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Aufsatznummer | 195 |
Seitenumfang | 7 |
Fachzeitschrift | Pathogens |
Jahrgang | 10 |
Ausgabenummer | 2 |
Publikationsstatus | Veröffentlicht - 12 Feb. 2021 |
Peer-Review-Status | Ja |
Externe IDs
PubMed | 33673032 |
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PubMedCentral | PMC7917696 |
ORCID | /0000-0003-4963-7523/work/142242900 |
ORCID | /0000-0003-1526-997X/work/142247236 |
ORCID | /0000-0002-9301-1803/work/161409763 |
Schlagworte
Ziele für nachhaltige Entwicklung
ASJC Scopus Sachgebiete
Schlagwörter
- Monitoring, SARS-CoV-2, Surveillance, Virus concentration, Wastewater, Wastewater-based epidemiology