Embryonic Tissue and Blastema Transplantations

Research output: Contribution to book/conference proceedings/anthology/reportChapter in book/anthology/reportInvited

Contributors

Abstract

Embryo grafts have been an experimental pillar in developmental biology, and particularly, in amphibian biology. Grafts have been essential in constructing fate maps of different cell populations and migratory patterns. Likewise, autografts and allografts in older larvae or adult salamanders have been widely used to disentangle mechanisms of regeneration. The combination of transgenesis and grafting has widened even more the application of this technique.In this chapter, we provide a detailed protocol for embryo transplants in the axolotl (Ambystoma mexicanum ). The location and stages to label connective tissue, muscle, or blood vessels in the limb and blood cells in the whole animal. However, the potential of embryo transplants is enormous and impossible to cover in one chapter. Furthermore, we provide a protocol for blastema transplantation as an example of allograft in older larvae.

Details

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages235-247
Number of pages13
Volume2562
Publication statusPublished - 2023
Peer-reviewedNo

Publication series

SeriesMethods in molecular biology (Clifton, N.J.)
ISSN1064-3745

External IDs

Scopus 85140349281
unpaywall 10.1007/978-1-0716-2659-7_16
Mendeley c7ed2683-520b-346b-9d36-1dd1f1470c8e

Keywords

DFG Classification of Subject Areas according to Review Boards

Subject groups, research areas, subject areas according to Destatis

Sustainable Development Goals

ASJC Scopus subject areas

Keywords

  • Animals, Ambystoma mexicanum/physiology, Extremities/physiology, Connective Tissue, Larva/physiology, Blood Vessels, Blood Cells, Axolotl Embryos, Lateral Plate Mesoderm, Presomitic Mesoderm, Muscle