The spectral properties of two spherical metallic nanoparticles of 80 nm in diameter are examined with regard to the interparticle distance and relative polarization of the excitation light. One Au nanoparticle is attached to a scanning fiber probe and the second to a scanning substrate. This configuration allows three-dimensional and arbitrary manipulation of both distance and relative orientation with respect to the incident light polarization. As supported by numerical simulations, a periodic modulation of the coupled plasmon resonance is observed for separations smaller than 1.5 μm. This interparticle coupling affects the scattering cross section in terms of spectral position and spectral width as well as the integral intensity of the Mie-scattered light.