Current practices in the study of biomolecular condensates: a community comment

Research output: Contribution to journalComment/DebateContributedpeer-review

Contributors

  • Simon Alberti - , Chair of Cellular Biochemistry (Author)
  • Paolo Arosio - , ETH Zurich (Author)
  • Robert B Best - , National Institutes of Health (NIH) (Author)
  • Steven Boeynaems - , Baylor College of Medicine (Author)
  • Danfeng Cai - , Johns Hopkins University (Author)
  • Rosana Collepardo-Guevara - , University of Cambridge (Author)
  • Gregory L Dignon - , Rutgers - The State University of New Jersey, New Brunswick (Author)
  • Rumiana Dimova - , Max Planck Institute of Colloids and Interfaces (Author)
  • Shana Elbaum-Garfinkle - , City University of New York (Author)
  • Nicolas L Fawzi - , Brown University (Author)
  • Monika Fuxreiter - , University of Padua (Author)
  • Amy S Gladfelter - , Duke University (Author)
  • Alf Honigmann - , Chair of Biophysics (Author)
  • Ankur Jain - , Whitehead Institute for Biomedical Research (Author)
  • Jerelle A Joseph - , Princeton University (Author)
  • Tuomas P J Knowles - , University of Cambridge (Author)
  • Keren Lasker - , Scripps Research Institute (Author)
  • Edward A Lemke - , Institute of Molecular Biology (IMB) gGmbH (Author)
  • Kresten Lindorff-Larsen - , University of Copenhagen (Author)
  • Reinhard Lipowsky - , Max Planck Institute of Colloids and Interfaces (Author)
  • Jeetain Mittal - , Texas A&M University (Author)
  • Samrat Mukhopadhyay - , Indian Institute of Science Education and Research Mohali (Author)
  • Sua Myong - , Boston Children's Hospital (Author)
  • Rohit V Pappu - , Washington University St. Louis (Author)
  • Karsten Rippe - , Heidelberg University  (Author)
  • Tatyana A Shelkovnikova - , University of Sheffield (Author)
  • Anthony G Vecchiarelli - , University of Michigan, Ann Arbor (Author)
  • Susanne Wegmann - , German Center for Neurodegenerative Diseases (DZNE) - - Partner Site Berlin (Author)
  • Huaiying Zhang - , Carnegie Mellon University (Author)
  • Mingjie Zhang - , Southern University of Science and Technology (Author)
  • Chloe Zubieta - , French National Centre for Scientific Research (CNRS) (Author)
  • Markus Zweckstetter - , German Center for Neurodegenerative Diseases (DZNE) - Partner Site Göttingen (Author)
  • Dorothee Dormann - , Institute of Molecular Biology (IMB) gGmbH (Author)
  • Tanja Mittag - , St. Jude Children Research Hospital (Author)

Abstract

The realization that the cell is abundantly compartmentalized into biomolecular condensates has opened new opportunities for understanding the physics and chemistry underlying many cellular processes, fundamentally changing the study of biology. The term biomolecular condensate refers to non-stoichiometric assemblies that are composed of multiple types of macromolecules in cells, occur through phase transitions, and can be investigated by using concepts from soft matter physics. As such, they are intimately related to aqueous two-phase systems and water-in-water emulsions. Condensates possess tunable emergent properties such as interfaces, interfacial tension, viscoelasticity, network structure, dielectric permittivity, and sometimes interphase pH gradients and electric potentials–. They can form spontaneously in response to specific cellular conditions or to active processes, and cells appear to have mechanisms to control their size and location–. Importantly, in contrast to membrane-enclosed organelles such as mitochondria or peroxisomes, condensates do not require the presence of a surrounding membrane.

Details

Original languageEnglish
Article number7730
JournalNature communications
Volume16
Issue number1
Publication statusPublished - 19 Aug 2025
Peer-reviewedYes

External IDs

PubMedCentral PMC12365244
Scopus 105013782623
ORCID /0000-0003-4017-6505/work/191040359
ORCID /0000-0003-0475-3790/work/191041723

Keywords