Creatine Is a Scavenger for Methylglyoxal under Physiological Conditions via Formation of N-(4-Methyl-5-oxo-1-imidazolin-2-yl)sarcosine (MG-HCr)
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Contributors
Abstract
Following incubation of methylglyoxal and creatine under physiological conditions, N-(4-methyl-5-oxo-1-imidazolin-2-yl)sarcosine (MG-HCr) was isolated and identified by NMR and mass spectrometry. Due to its rapid formation, MG-HCr represents a specific product following "scavenging" of methylglyoxal by creatine. Using hydrophilic interaction chromatography coupled to mass spectrometry, MG-HCr was analyzed in urine samples of healthy volunteers. Daily MG-HCr excretion of nonvegetarians ranged from 0.35 to 3.84 mu mol/24 h urine (median: 0.90 mu mol/24 h urine) and of vegetarians from 0.11 to 0.31 mu mol/24 h urine (median: 0.19 mu mol/24 h urine), indicating that formation of MG-HCr in vivo is influenced by the dietary intake of creatine. The trapping of methylglyoxal by creatine may delay the formation of advanced glycation compounds in vivo and, therefore, could be of special importance in situations in which the body has to deal with pathophysiologically increased amounts of dicarbonyl compounds ("carbonyl stress"), for instance in diabetic patients.
Details
Original language | English |
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Pages (from-to) | 2249-2256 |
Number of pages | 8 |
Journal | Journal of agricultural and food chemistry |
Volume | 63 |
Issue number | 8 |
Publication status | Published - 4 Mar 2015 |
Peer-reviewed | Yes |
External IDs
WOS | 000350615000016 |
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Scopus | 84924267842 |
Keywords
Sustainable Development Goals
Keywords
- methylglyoxal, creatine, dicarbonyl compounds, glycation, carbonyl stress, diabetes, meat, ADVANCED GLYCATION ENDPRODUCTS, DILUTION ANALYSIS, END-PRODUCTS, DIETARY, PROTEIN, 3-DEOXYGLUCOSONE, AMINOGUANIDINE, IDENTIFICATION, METABOLITE, ARGININE