Core PCP mutations affect short-time mechanical properties but not tissue morphogenesis in the Drosophila pupal wing

Research output: Contribution to journalResearch articleContributedpeer-review

Contributors

  • Romina Piscitello-Gómez - , Chair of Systems Biology and Genetics, Max Planck Institute of Molecular Cell Biology and Genetics, TUD Dresden University of Technology (Author)
  • Franz S. Gruber - , Max Planck Institute of Molecular Cell Biology and Genetics, University of Dundee (Author)
  • Abhijeet Krishna - , Chair of Systems Biology and Genetics, Clusters of Excellence PoL: Physics of Life, Max Planck Institute of Molecular Cell Biology and Genetics, Center for Systems Biology Dresden (CSBD) (Author)
  • Charlie Duclut - , Institut Curie, Max-Planck-Institute for the Physics of Complex Systems (Author)
  • Carl D. Modes - , Max Planck Institute of Molecular Cell Biology and Genetics, TUD Dresden University of Technology, Center for Systems Biology Dresden (CSBD) (Author)
  • Marko Popović - , TUD Dresden University of Technology, Center for Systems Biology Dresden (CSBD), Max-Planck-Institute for the Physics of Complex Systems (Author)
  • Frank Jülicher - , TUD Dresden University of Technology, Center for Systems Biology Dresden (CSBD), Max-Planck-Institute for the Physics of Complex Systems (Author)
  • Natalie A. Dye - , Biophysics of epithelial growth and tumorigenesis (NFoG), Max Planck Institute of Molecular Cell Biology and Genetics (Author)
  • Suzanne Eaton - , Biotechnology Center, Chair of Developmental Cell Biology, Max Planck Institute of Molecular Cell Biology and Genetics, Center for Systems Biology Dresden (CSBD) (Author)

Abstract

How morphogenetic movements are robustly coordinated in space and time is a fundamental open question in biology. We study this question using the wing of Drosophila melanogaster, an epithelial tissue that undergoes large-scale tissue flows during pupal stages. Previously, we showed that pupal wing morphogenesis involves both cellular behaviors that allow relaxation of mechanical tissue stress, as well as cellular behaviors that appear to be actively patterned (Etournay et al., 2015). Here, we show that these active cellular behaviors are not guided by the core planar cell polarity (PCP) pathway, a conserved signaling system that guides tissue development in many other contexts. We find no significant phenotype on the cellular dynamics underlying pupal morphogenesis in mutants of core PCP. Furthermore, using laser ablation experiments, coupled with a rheological model to describe the dynamics of the response to laser ablation, we conclude that while core PCP mutations affect the fast timescale response to laser ablation they do not significantly affect overall tissue mechanics. In conclusion, our work shows that cellular dynamics and tissue shape changes during Drosophila pupal wing morphogenesis do not require core PCP as an orientational guiding cue.

Details

Original languageEnglish
Article numbere85581
JournaleLife
Volume12
Publication statusPublished - 2023
Peer-reviewedYes