Core PCP mutations affect short-time mechanical properties but not tissue morphogenesis in the Drosophila pupal wing

Publikation: Beitrag in FachzeitschriftForschungsartikelBeigetragenBegutachtung

Beitragende

  • Romina Piscitello-Gómez - , Professur für Systembiologie und Genetik, Max Planck Institute of Molecular Cell Biology and Genetics, Technische Universität Dresden (Autor:in)
  • Franz S. Gruber - , Max Planck Institute of Molecular Cell Biology and Genetics, University of Dundee (Autor:in)
  • Abhijeet Krishna - , Professur für Systembiologie und Genetik, Exzellenzcluster PoL: Physik des Lebens, Max Planck Institute of Molecular Cell Biology and Genetics, Zentrum für Systembiologie Dresden (CSBD) (Autor:in)
  • Charlie Duclut - , Institut Curie, Max-Planck-Institute for the Physics of Complex Systems (Autor:in)
  • Carl D. Modes - , Max Planck Institute of Molecular Cell Biology and Genetics, Technische Universität Dresden, Zentrum für Systembiologie Dresden (CSBD) (Autor:in)
  • Marko Popović - , Technische Universität Dresden, Zentrum für Systembiologie Dresden (CSBD), Max-Planck-Institute for the Physics of Complex Systems (Autor:in)
  • Frank Jülicher - , Technische Universität Dresden, Zentrum für Systembiologie Dresden (CSBD), Max-Planck-Institute for the Physics of Complex Systems (Autor:in)
  • Natalie A. Dye - , Biophysik von epithelialen Wachstum und Tumorentstehung (NFoG), Max Planck Institute of Molecular Cell Biology and Genetics (Autor:in)
  • Suzanne Eaton - , Biotechnologisches Zentrum (BIOTEC), Professur für Entwicklungszellbiologie (gB MPI-CBG), Max Planck Institute of Molecular Cell Biology and Genetics, Zentrum für Systembiologie Dresden (CSBD) (Autor:in)

Abstract

How morphogenetic movements are robustly coordinated in space and time is a fundamental open question in biology. We study this question using the wing of Drosophila melanogaster, an epithelial tissue that undergoes large-scale tissue flows during pupal stages. Previously, we showed that pupal wing morphogenesis involves both cellular behaviors that allow relaxation of mechanical tissue stress, as well as cellular behaviors that appear to be actively patterned (Etournay et al., 2015). Here, we show that these active cellular behaviors are not guided by the core planar cell polarity (PCP) pathway, a conserved signaling system that guides tissue development in many other contexts. We find no significant phenotype on the cellular dynamics underlying pupal morphogenesis in mutants of core PCP. Furthermore, using laser ablation experiments, coupled with a rheological model to describe the dynamics of the response to laser ablation, we conclude that while core PCP mutations affect the fast timescale response to laser ablation they do not significantly affect overall tissue mechanics. In conclusion, our work shows that cellular dynamics and tissue shape changes during Drosophila pupal wing morphogenesis do not require core PCP as an orientational guiding cue.

Details

OriginalspracheEnglisch
Aufsatznummere85581
FachzeitschrifteLife
Jahrgang12
PublikationsstatusVeröffentlicht - 2023
Peer-Review-StatusJa